Pdf identification of hortaea werneckii isolated from. Hortaea werneckii is a species of yeast in the family teratosphaeriaceae. B15, isolated from petroleumcontaminated soil for biodegradation of phenanthrene. Download product flyer is to download pdf in new tab. The mycological and molecular study of hortaea werneckii. Ecology and molecular adaptations of the halophilic black. In the d1d2 domains of 28s rdna, 10 genotypes aj were recognised with the manual inspection of the sequence alignment, as also seen in. It is an infrequent asymptomatic infection that affects mainly human palms and soles, and it is mostly seen in tropical countries. Global molecular diversity of the halotolerant fungus hortaea werneckii. Life free fulltext global molecular diversity of the. Pdf hortaea werneckii is an example of phaeoid dematiaceousfungi. Tinea nigra by hortaea werneckii, a report of 22 cases from mexico. These yeasts were unambiguously identified by phenotypic and genotypic methods. Hortaea werneckii is more frequently found in eutrophic thalassohaline waters at moderate temperatures, while it is not known to occur in oligotrophic or athalasso.
Genomic adaptations of the halophilic dead sea filamentous. For full access to this pdf, sign in to an existing account. Full length article characterization of new strains of hortaea werneckii isolated from salt marshes of egypt ashraf elsayed, amr m. Tinea nigra by hortaea werneckii, a report of 22 cases.
Tinea nigra is an uncommon superficial dermatomycosis usually caused by hortaea werneckii, formerly known as phaeoannellomyces werneckii, formerly classified as exophiala werneckii and cladosporium werneckii. It represents a new model organism for studying the mechanisms of salt tolerance in. Hortaea werneckii, polyethylene plastic, scuba diving, silicone rubber, tinea nigra. A global set of clinical and environmental strains of the halotolerant black yeast like fungus hortaea werneckii are analyzed by. We evaluate retrospectively twentytwo confirmed cases of. Identification and characterization of ena atpases hwena1. The extremely halotolerant black yeast hortaea werneckii.
It is an infrequent, asymptomatic infection, limited to tropical and subtropical countries. Download fulltext pdf download fulltext pdf tinea nigra by hortaea werneckii, a report of 22 cases from mexico article pdf available in studies in mycology 61. Hortaea werneckii an overview sciencedirect topics. Hortaea werneckii is a black yeast recently isolated from salterns in slovenia.
Hortaea werneckii is a common saprophytic fungus believed to occur in soil, compost, humus and on wood in humid tropical and subtropical regions and is the causative agent of tinea nigra in humans rg1 organism. One excellent example of a fungal extremophile is the halophile hortaea werneckii pezizomycotina, dothideomycetes, capnodiales, a yeastlike fungus able to thrive at nearsaturating concentrations of sodium chloride and which is also tolerant to both uv irradiation and desiccation. Some of the adaptations of halophilic microorganisms to increased salinity and. For questions about the data at this site, please contact its webmaster. Abstracttinea nigra is a rare superficial mycosis caused by hortaea werneckii. Now, this fungus has been found in the halophilic environment all over the world and recognized as a new model organism in exploring the mechanisms of salt tolerance in eukaryotes. To emphasize this unusually wide adaptability, and to distinguish h. Pdf tinea nigra is a superficial mycosis caused by hortaea werneckii. Adaptation of extremely halotolerant black yeast hortaea werneckii to increased osmolarity.
Tinea nigra is a superficial mycosis caused by hortaea werneckii. Unique characteristics of the key map kinases of the hog pathway help the fungus hortaea werneckii to survive and proliferate in hyperosmolar environments, like. Hortaea werneckii, the causative agent of tinea nigra in people, was diagnosed in a household guinea pig in japan sharmin et al. Magdy botany department, faculty of science, mansoura university, mansoura, egypt. Hortaea werneckii is an environmental dematiaceous fungus found in the halophilic environment. The halophilic fungus hortaea werneckii and the halotolerant fungus aureobasidium pullulans maintain low intracellular cation concentrations in hypersaline environments. A homolog of the hog1 gene was isolated from the halophilic black yeast hortaea werneckii encoding a putative 359 amino acid protein, hwhog1p, with high homology to saccharomyces cerevisiae hog1p and to other eukaryotic hog1p homologs. This aim of the study is to investigate a halophilic bacterium hortaea sp.
Kogej t 2006 physiological adaptations of halophilic black yeast hortaea werneckii to growth at saline conditions on the levels of cell wall and accumulation of compatible solutes. Hortaea werneckii is one of the most salttolerant species among microorganisms. Special attention was paid to the wall architecture, the septum with a simple pore apparatus, the annellidic type of collar and the nuclei. It is not permitted to download or to forwarddistribute the text or part of it without the consent of the authors andor holders, other than for strictly personal, individual use, unless the work is under an open content license like creative commons. Hortaea werneckii isolated from silicone scuba diving. Exophiala is a genus of anamorphic fungi in the family herpotrichiellaceae. A global set of clinical and environmental strains of the halotolerant black yeastlike fungus hortaea werneckii are analyzed by multilocus sequencing and aflp, and physiological parameters are determined. Role of oxidative stress in the extremely salttolerant. Hortaea werneckii is the etiologic agent of tinea nigra, a superficial cutaneous mycosis typically involving either the palms. However, the black yeast hortaea werneckii has been identified as the dominant fungal species in hypersaline waters on three continents. It is an infrequent asymptomatic infection that affects human palms and soles, and is mostly observed in tropical countries.
We evaluate retrospectively twentytwo confirmed cases of tinea nigra from a total of eleven yr 19972007 and discuss the epidemiology, clinical. Global molecular diversity of the halotolerant fungus hortaea. Hortaea werneckii was reported to cause tinea nigra in humans. Hortaea werneckii is a melanised yeastlike fungus, belonging to. Exophiala has been implicated in causing saxophone lung or hypersensitivity pneumonitis, a disease that can be contracted by woodwind instrumentalists saxophonists, clarinettists, oboists etc. Identification and characterization of ena atpases hwena1 and hwena2 from the halophilic. Pdf global molecular diversity of the halotolerant. The term mycoses refers to infections that are caused by fungi. Biodegradation mechanism of phenanthrene by halophilic. The fine structure of hortaea werneckii, mycoses 10. Adaptation of extremely halotolerant black yeast hortaea.
In the present study we assessed their growth and determined the intracellular cation concentrations of saltadapted and nonsaltadapted cells of both. Pdf tinea nigra by hortaea werneckii, a report of 22. Hortaea werneckii is a saprophytic fungus which can be found in tropical and subtropical soils, compost, and decaying wood. Tinea nigra may also be due to stenella araguata, first described and named cladosporium castellanii in 1973. A new meristematic fungus westerdijk fungal biodiversity institute. This fungus is also halophilic as it has been isolated from salted foods, saline waters and natural saltpans, growing in salt concentrations of up to 10%.
The black yeast hortaea werneckii mzki b736 was from the culture collection of the slovenian national institute of chemistry. Compatible solutes, differential gene expression, hal2, halophile, hog signaling pathway, hortaea werneckii, hypersaline water, ions, melanin. The metabolitic product of phenanthrene was identified and assayed by using ultravioletvisible. The map kinase hwhog1 from the halophilic black yeast. Andrea kalus, in the travel and tropical medicine manual fifth edition, 2017. Some of the adaptations of halophilic microorganisms to increased salinity and osmolarity of the environment are alterations in membrane properties. Hortaea werneckii is a black yeastlike ascomycetous fungi associated with the human superficial infection tinea nigra, which commonly occurs in tropical and subtropical countries. The project works to allow users to contribute quality articles and media files to the encyclopedia and track their progress as they are developed. Lesions included a focal area of ulceration and alopecia on the back and black pigmentation on the palmar aspect of. Tinea nigra is a superficial dermatomycosis precipitated by hortaea werneckii, a melanized yeastlike fungus formerly classified in the literature as exophiala, phaeoannellomyces, and cladosporium. Bilateral tinea nigra plantaris with good response to isoconazole. Osmoadaptationdependent activity of microsomal hmgcoa. B15 has the ability to completely degrade phenanthrene 100 mgl under salinity 10% within 1week incubation.
The mitogenactivated protein kinase mapk hog1p plays an essential role in the yeast hyperosmotic response. Download pdf, adaptation of extremely halotolerant black yeast hortaea werneckii to increase osmolarity. Phylogenetic tree constructed by mega using neighborjoining tree, with number of bootstrap replications 500, between different hortaea werneckii strains including the three isolates and phaeotheca triangularis. During a survey of black yeasts of marine origin, some isolates of hortaea werneckii were recovered from scuba diving equipment, such as silicone masks and snorkel mouthpieces, which had been kept under poor storage conditions.
The mycoses with the highest incidencecandidiasis and dermatophytosisare caused by fungi that are frequent components of the normal human microbiota and highly adapted to survival on the human host. A global set of clinical and environmental strains of the halotolerant black yeastlike fungus hortaea werneckii are analyzed by. Molecular studies on halophilic adaptations have focused on prokaryotic microorganisms due to a lack of known appropriate eukaryotic halophilic microorganisms. Adaptation of the glycerol3phosphate dehydrogenase gpd1 to high salinities in the extremely halotolerant hortaea werneckii and halophilic. Uvadare digital academic repository tinea nigra by. Hortaea werneckii and aureobasidium pullulans, black yeastlike fungi isolated from hypersaline waters of salterns as their natural ecological niche, have been previously defined as halophilic and halotolerant microorganisms, respectively. Characterization of new strains of hortaea werneckii. Salt stress affects sterol biosynthesis in the halophilic. Hortaea werneckii isolated from silicone scuba diving equipment in. Most pathogenic fungi are exogenous, their natural habitats being water, soil, and organic debris. This pathogen thrives in hightemperature, lowoxygen, and humid environments, affecting individuals in temperate or subtropical climates.
Its primary ecological niche are natural hypersaline environments worldwide. Pdf tinea nigra by hortaea werneckii, a report of 22 cases from. Pdf identification of hortaea werneckii isolated from mangrove. The extremely halotolerant black yeast hortaea werneckii a model. The ascomycetous black yeast hortaea werneckii capnodiales, teratosphaeriaceae is the most extremely halotolerant fungus known, and as such, it has become an important model organism for the study of halotolerance in eukarya plemenitas et al. Lesions included a focal area of ulceration and alopecia on the back and black pigmentation on the palmar aspect of the right forepaw. While the addition of salt to the medium is not required for its cultivation, h. For support of the browser software only, send email to gmod. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. It is a black yeast that is investigated for its remarkable halotolerance.
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